FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DAZ1_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DAZ1_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13667858
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG196570.1438191704947476No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA15400.012.27359459
TGGTCGC19350.010.13032255
GCCGTAT20100.010.10003160
GGTCGCC20300.09.82867556
CCGTATC23750.08.10550961
TGGGGGG48400.07.73834857
CGTATCA29500.06.999802662
TCGCCGT28200.06.95061758
TCGGTGG29250.06.82079551
GGCTATA94100.06.807368370
AGGCTAT98350.06.691157370
TAGGCTA111100.06.490391770
TGTAGGC126250.06.48787270
CTATAGT86000.06.308861770
GCTATAG90350.06.16010869
GTAGGCT119850.06.045770669
GTCGCCG32650.06.0036457
AGTGTAG198150.05.95324370
GAGTGTA183550.05.377897370
TATAGTG77750.05.177441670