FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_DAZ1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_DAZ1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13702189
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG197610.1442178326397337No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA14300.011.50436259
TGGTCGC18250.09.78180955
GCCGTAT17200.09.76810660
GGTCGCC19100.08.613276556
AGGCTAT98150.07.16832470
CCGTATC22900.06.878146661
TAGGCTA110600.06.80448470
GGCTATA92800.06.6763270
TGGGGGG50150.06.56083757
TGTAGGC128550.06.507852670
GTAGGCT120800.06.229978669
CTATAGT85750.05.959784570
AGTGTAG193550.05.877670369
GAGTGTA182000.05.846743670
TATAGTG77250.05.845292669
TCGCCGT28950.05.803397758
GCTATAG91650.05.72893469
TCGGTGG30550.05.728473751
GTCGCCG31750.05.622491457
CGTATCA29000.05.55169762
Produced by FastQC (version 0.11.5)