FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_ColSPL8_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_ColSPL8_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences995159
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG12410.12470369056603015No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGCAG1350.002944766515.55671661
CGTCCCC1400.003751583915.00112168
TCGGACT1750.001013343114.0080875
CCGTCCC1550.007369858713.54939967
GGACTCT1950.00229600512.56693657
GCCGTGT2050.003344004711.95211269
CCCAGTC2150.004773046411.39619961
CAGTCCG2150.004774836411.39562663
TCTCGTG2650.001866718610.56788958
GCCGTTC2800.002950207610.00124955
ACAGACA2950.0045473519.4912845
CCCGAGA3100.0068392769.03202446
CGTTCCC3200.0088577578.75153357
TTCCCAG3200.0088577578.75153359
GATTGAC4108.012694E-48.53636551
AGCTCCC4050.00681247057.777186442
GAGAGCC4150.0084697817.590164751
CGTGTAG9159.566029E-76.885760369
GTCGTGT9953.791738E-66.332131467
GTGTAGG9953.791738E-66.332131470
Produced by FastQC (version 0.11.5)