FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_ColSPL16_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_ColSPL16_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004342
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11870.11818683277210351No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCCG1550.007372174413.54877465
TCAGGAC1850.001547652513.2436251
ACTAAGC2250.00670315910.88811416
AGAGCCG3350.00122083229.40418970
AATACAG4250.0011436588.23552852
CAGATAA4900.00462776147.14234914
GTTACAT5200.0082355946.72995529
AGAGTGT7600.0084239135.52674866
GTGTAGG9606.1217253E-45.469450569
CGTGTAG9400.00278151275.21317468
GAGCGTC10905.997163E-45.13724962
TGTAGGG9800.0046722475.000640470
GTCGTGT10500.00202282635.00039166
Produced by FastQC (version 0.11.5)