FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_ColSPL13_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_ColSPL13_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1605357
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG23190.14445385045195555No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGGCC950.007201322418.4257033
CCTGGGG1600.00909793713.12504117
CGTCGCT2800.002952583610.00096656
GACCTGA3208.0360234E-49.84439559
TGAGAGC4450.00180811047.86274438
CGAGTTT6853.6808892E-46.642356416
CAGAGCC5850.00349352766.581832468
TCAGAGC6200.0064162946.20911663