FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_ColSPL10_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_ColSPL10_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2734771
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG41320.1510912613889792No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGCGCA400.002420048234.999914
CGGACTG2607.872723E-613.4666736
GTCAGCG1600.00910043713.12496314
CGGACTA2800.002946757510.0038146
CCGGACT3652.499412E-49.5930495
TCCGGAC4159.0087106E-48.4374154
TCGTCGC4000.00607640347.8780046
GAGCCGT9009.325959E-87.38940870
GGACTGT4750.00340959767.3706927
AGAGCCG9251.149534E-66.8112970
CAGAGCC10954.1112798E-76.393143769
ACACGAA7500.00729875175.59998414
TCAGAGC12554.6797213E-65.57777568
GTCGACA9150.00198811395.35556869
GTTCAGA16002.3064258E-65.03132866
CGTGTAG31500.05.000351469
Produced by FastQC (version 0.11.5)