FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_COLSPL8_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_COLSPL8_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2701539
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG28180.10431091314987494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGACC6101.0686563E-89.7617641
ACTGCAC5603.188179E-79.379762
AACCCCC5754.569765E-68.5284361
CCGGCCA3950.00545845657.972675336
ACCCCCT4050.00678450337.7817272
GGGAGTC5502.189931E-47.636137515
AACCACT17450.07.4270351
AACTGCA25600.07.11496161
AACCGTG10058.549068E-86.9706481
TGTAGGC8652.9259481E-66.879300668
AACCAGC8158.838775E-66.87656551
AACCCAC8703.1683012E-66.8444551
GACACTA5250.009035656.66646912
GTAGGCT7904.2694686E-56.646357569
AACTACC14005.657057E-106.5051081
AACTGTC17301.8189894E-126.4790761
ACCGATA7105.661549E-46.4117042
AACTCCC7651.9985311E-46.41026261
AACTTCC21200.06.2785151
AACGACA8409.8843404E-56.2549121
Produced by FastQC (version 0.11.5)