FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_COLSPL13_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_COLSPL13_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2130250
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG26300.12345968783006689No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAG1951.4125979E-414.3621356
CCGCTGG2408.170897E-411.66731616
AGGGTGT5250.0090284326.66688170
GGATAGG7055.2246894E-46.45410970
GTAGGAT11401.2455712E-76.44757669
GATAGGG6200.00641049176.20987770
TCACTAC6400.0089323316.01412435
TAGGATA14858.850293E-85.65674870
TGTAGGA13857.9194797E-75.559746368
AGTGTAG13302.211218E-65.52636466
GTGTAGG28000.05.25016969
GAGTGTA13451.571456E-55.20426265
Produced by FastQC (version 0.11.5)