FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_COLSPL10_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_COLSPL10_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences82
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCGTAAGAAAAATTAACTCGTTAATTTTTTTTGAATATTTTCGTTTTTTT22.4390243902439024No Hit
ATTAGGGAAACTTCCTGTGACTGGAACATTAATGGTCAAATGCACTTTGC22.4390243902439024No Hit
TTGCATAGTTACTCTTACGTAGAACCGCAGTATCGGATGTCGAGTTCGTC11.2195121951219512No Hit
AAGTAACTTTCTCACCTAAAGCATATGCATACATGAAATCTTCCAAATCT11.2195121951219512No Hit
AATTCCTGCCAATAACCACGCCAGGAAATTAAGAACATAAATCCTGTAGC11.2195121951219512No Hit
AAGTGAGTTCATAACCATTGCATTGCTTGATCCACTTTTCGTGCTTAATG11.2195121951219512No Hit
TGGAAGGTAAAAAGGTTATTATTAGGATCTATTTTACTTATAAGACGACA11.2195121951219512No Hit
GAGTTGGAAGAATTGGAAGGCGAAAATGAAGAAAATGTACCAATGGAGCC11.2195121951219512No Hit
TACATTTCGTTCTACGAACAAGAAGAAGATCACGATAAGATGCTACTCAG11.2195121951219512No Hit
ATTATAAAAGCTATCTTAAATGATAAATATCAGAACTCCACGAAAAAAAT11.2195121951219512No Hit
AGTTTTTATCCAAGCTATGAACTGCATCAGATGCCTTTTAAAGTCTATTA11.2195121951219512No Hit
TTAACATAAAACAATTCAAACATGTCCAGTAGGTGGTTTAGTGCATCAGG11.2195121951219512No Hit
TGTTATCGAGCCGTTTTATTAGGAATAACTCGAGCATCTTTGAATACTCA11.2195121951219512No Hit
TTTGTGTCCGATGATCGTTGATTGGTTTCTCAGGGTTTAAGTTGGTGTTG11.2195121951219512No Hit
AAACAACTTATTCAAATAAACATAAGTTCAAGTAGAGTTTGGGCAAAGAT11.2195121951219512No Hit
GGGGAGACTTGTAACGCCCGCGATCCTCAATAGGATCGTTGGCTGGGACT11.2195121951219512No Hit
TCTTGCTAACACCAATATTGAACTTCCTGGTAATATTTTTCATGATTCTT11.2195121951219512No Hit
TAACCATATTTTCTTGCTAATAAAGTAATAATCCCCTCTAATTTGAATTA11.2195121951219512No Hit
CGTTTCTTTTTTGGGGTCAATATCAGCAATGCAGTTCATCCAACGATAAA11.2195121951219512No Hit
CAAAGCGGCTCTATTTCATTATATTCCATCCATATCCCAATTCCATTCAT11.2195121951219512No Hit
AGAAACTCTGTTATAGCCATTTCTGTACATTCAATGTACTCTACGGATAG11.2195121951219512No Hit
TTATTGCTGGTTAGACAAAACATGAAACGAATACAAAAACGCACAAATTA11.2195121951219512No Hit
ATACCAGTTTATGATCATAAAACGCACAACGGTTAAGAAGAAAATTGAGA11.2195121951219512No Hit
CCACACAGTTGTCCATGTACCAGTAGAAGATTCAGCAGCTACCGCAGCCC11.2195121951219512No Hit
AATGAAGACATATATAAAAGTAGAAAATATATAAATATGATATACAAAAT11.2195121951219512No Hit
AAAAACATAATAATACAGCACCCATATATTCTTGACGATCCATTTGATGC11.2195121951219512No Hit
ATATAGAGAAAACAAGTACGAAGTTCAATTTGAAATAAAAAAATTGAAAG11.2195121951219512No Hit
TGTTATTTCCAAATTATCCTCTCATTTGCAAATATTATAACATCATTAGA11.2195121951219512No Hit
TAGTGTACCGTAGTAGACAGTACCGAAGCCTCCATCTCCGAGTTCTTTGG11.2195121951219512No Hit
TGTATTCTGTATAGTTATCTAGACGAGTTTAAGAAGGTGATAAGATTCTA11.2195121951219512No Hit
AGAAATATTATATGTTTGTATTTTACTATCATGATTTATGAAAAATTCTA11.2195121951219512No Hit
ATTTTACAATATTACAAAATACCTGAAAATAAACATTTGATCATGAATTA11.2195121951219512No Hit
ACATTATGAAACAGTAATCGCTATCTATCATAACGTCCATTGAATTTTTG11.2195121951219512No Hit
GCTGAAATCGAGAATCTTGAAGCTAATGAGACCAGAGAATTCAATATTAC11.2195121951219512No Hit
CACACGTTATTTGTCTGCAGCCAACCCTCGGCAACAAACTATATATACAC11.2195121951219512No Hit
AGTACTCGTTTAATCTCCTCCTCTGTTACTGACCTAGTCATCTCTGTTTT11.2195121951219512No Hit
AACATCAAAACTAAATAAGCAAAAAAAACAACATAACAGACCTCAACGTA11.2195121951219512No Hit
TCTTTCTCTCCCCCAGACTTATTATTTTTTAGAAAGTAAACAATGGGGAA11.2195121951219512No Hit
TTGATGGTTTAGCCGAATTCCGTATGAGTCTTTGTCTATGTATCTTCTAA11.2195121951219512No Hit
AGTTGATAGTATTAGATTCTTTATACGCCGGCGTGGGGATGCGCAGGCGC11.2195121951219512No Hit
TGGATTCGTTTGGTCAGAAGTAAAAGAACTTTCTGATTTTTTGTCCAGGT11.2195121951219512No Hit
CTTCATCTTTTCTTATTGCAGAGAAAAACTCAGTGTCGATCGATACACCA11.2195121951219512No Hit
GAGATATGAAGAAGCATCCTCGTCCCACGGTTTACATTTCACCAAAACCG11.2195121951219512No Hit
TACAAAGATCGGGATTTTGCTGGATGGTTAAAATACTATGTCAGTATATG11.2195121951219512No Hit
AAGATTTCATGGTTTAGCCAAAGTCCATATGAGTCTTTGGCTTTGGTCTT11.2195121951219512No Hit
AACCAGCTTCTTGGATTATCATTTAATCTAAACAATAGTATAGTTAATAG11.2195121951219512No Hit
ATAATTGTGGAATATATATTGGACTACGTAAAAGCCCAAAACAAAATATA11.2195121951219512No Hit
GTGCTTGGGCGAGAGTAGTACTAGGATGGGTGACCTCCCGGGAAGTCCTC11.2195121951219512No Hit
AAACACAAAACACTTGAAATATCTTCTTGAAAAAAGGAAGTCGTTTGAGA11.2195121951219512No Hit
CTTGCTTCCTCAACCATATGGCTTTTATATCTTCCTTCGTGAGAGCACTA11.2195121951219512No Hit
CAAATATTTCCGCAAGACCAGATCAAACATTGCAAAATATATATACAAGT11.2195121951219512No Hit
TGATAAAATTCAAAACGTGTTTCTCTTTAACTTTTCCACTGGGAACCGAG11.2195121951219512No Hit
GGAGAGCATTTCAGAAAGCTTTGGGAACCAAGGTGCACTTGAGTAAAGCA11.2195121951219512No Hit
CCTAAGTTTCCTTATTTCATCAAATTTCCTGTTAGCAGGATCGGGTAGGA11.2195121951219512No Hit
TGTTAGCGACAAATAAATGAAAATATATAGATAAACCCAATAACAATATT11.2195121951219512No Hit
AATTATACATTGTTGAAAGCTTTTTTAATTAATTGAAATATAATCAAATT11.2195121951219512No Hit
GGGTTCATGTAGACGAGCTAGTTCCTGAGTTTTCCTATGTGTTTGGAGGA11.2195121951219512No Hit
AATTTTTCTTGAGCATGCATAAAAAAATTTTATCTCATATGATTAAAAAG11.2195121951219512No Hit
TTCTTATAAAGGAAAGAGTAGATCCATTACTCAACGCAGTGATGATGACA11.2195121951219512No Hit
TTAATTGAAATCATATTCTTGTGAAATAGCGGAGAAAACAGAATGTACAG11.2195121951219512No Hit
TTTTTATTTTGTCCGACAAAAGAACAAACTACGTATTTAGAGCGTGTCAT11.2195121951219512No Hit
ACAATACATCAATAACCTATTAATTAAACTTTGTAAAGATAGTAAAAATG11.2195121951219512No Hit
ATGATAAATATTAAAGGAGCAAAAAATCAATTCAAATTTCAATCTCATCC11.2195121951219512No Hit
AAAGAATTTGACTAACTGTGAAAAGAGAAAGGGGTCTCGCGGTAGATCGC11.2195121951219512No Hit
AGGATATATAAGTTAACAAACGAATGGTTCAACCATTTTATTAAACACTT11.2195121951219512No Hit
GTCATGTGTATGATTGAGTATAAGAACTTAAATCGCAACACGATCTTATA11.2195121951219512No Hit
AATAATGCAGACAAACTATTATTTTTTCTTTCTATTTGGCTAATATAATG11.2195121951219512No Hit
AATAAGGAAAGAGTTGACTCATATGGCTACCATTCTAACTACAAAAACTC11.2195121951219512No Hit
AGAAAGTAACACGCAAATAGAAGCCCCCACACAACTCATTTCCTCTAAAG11.2195121951219512No Hit
GGCTGCTTTGTTGAGCCGTCACACGGAATCGAGAGCTCCAAGTGGGCCAT11.2195121951219512No Hit
CGATACAACTCCTTATCTTCCAACAGTTCACCATCTGTCTTAAGTAACTT11.2195121951219512No Hit
GTACGATCTCTATAAATTAAACTCTCTCTTTTGATAAGTTTCATTAATTA11.2195121951219512No Hit
ATAATAAAAAAAACAAAAAAAATAACAAATTCTACACTAAATATTTGACA11.2195121951219512No Hit
AATAGAAGAATTCAATTTTCCTTCCTCATTCATCGGAGAGCTCTCTTTGG11.2195121951219512No Hit
TTTGTGTCTTCTAACAAGGATACAATTCTTACGCCTTTAAGATCCGGTTG11.2195121951219512No Hit
GCCCTATTGTTCCGATGGAGAGAAGAACCTATGATTAGCTTTTCAGGAAA11.2195121951219512No Hit
ATTTCTCTCATTTATAAGGTTCAAAATTTTTACATACTTTATACTAATTT11.2195121951219512No Hit
GAATGCTATATACTCCTTCCTTGTTTTTGCTGATAATGTCAGTTCCTCTT11.2195121951219512No Hit
AGGCCAAATTCCCGATTGATAGAATAATACGGTATGTATAAAAAAAAAGA11.2195121951219512No Hit
AATACTAACTGGAATCAAAGAATTAGTAGATCGGTTCCGCCCAAAATGGG11.2195121951219512No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAGAAA50.070.063
GAGAAAG50.070.064
TCCTTAT50.070.09
TTCATCA50.070.016
TAGGATA50.070.046
GTGTCAG50.070.057
AAATTTC50.070.022
TAGTGAG50.070.051
GAGTGTC50.070.055
TTTCCTT50.070.07
TTTCCTG50.070.025
CCTGTTA50.070.028
TAAGTTT50.070.03
AATTTCC50.070.023
AGGATCG50.070.037
AGGATAG50.070.047
GGGTAGG50.070.043
TCAGGAG50.070.060
CCTAAGT50.070.01
TTATTTC50.070.012
Produced by FastQC (version 0.11.5)