FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_C24SPL15m1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_C24SPL15m1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2594977
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32420.12493366993233466No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTACCG2950.00452645969.4976341
GCTCCGG3450.00159992829.13067469
GGGTCTC3750.0034093348.4002250
AGGGTGT7201.1675469E-57.29185870
GTAGGAT14700.07.14304470
TAGGGTG6753.0777772E-46.74091769
TGTAGGA18003.6379788E-126.22238569
GAGTGTA17002.2009772E-105.970744666
GATAGGG9701.13925635E-45.77334770
AGTGTAG17402.41198E-95.63233167
GGATAGG10152.1837532E-45.51738669
GGAGATC8900.00140263795.50576251
AGGATAG12501.46434E-45.04013268
Produced by FastQC (version 0.11.5)