FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_C24SPL11_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_C24SPL11_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2026828
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG25990.1282299238021184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTGTAG12753.4633558E-96.588909670
GCTATAG7206.74701E-46.319310765
TATAGTG8950.00150442795.4751567
AGAGTGT15552.3091161E-75.402481669
ATAGTGT10050.00113383185.22415768
GAGTGTA12803.8283742E-55.19584470
CTGTCTC8900.0083964765.11136834
Produced by FastQC (version 0.11.5)