FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_Al_SPL9_col_B_l2-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_Al_SPL9_col_B_l2-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1366
Sequences flagged as poor quality0
Sequence length76
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG241.7569546120058566No Hit
AGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTCTTCGCCTGTGTAGATC30.21961932650073207Illumina Single End PCR Primer 1 (96% over 33bp)
GGAACTGTGCTATCACACACCTTGGAAGAAAACGAAGTATTCCCTGCGCA20.14641288433382138No Hit
ACGGGACATTTGCTGAGTACGTTGATGTGTACTCATCCTTGCTTTACAGA20.14641288433382138No Hit
AAGTCTGCTCTACAGCGACGTAGATGCCCAGGCCTTTGAGGCTAAGCGCG20.14641288433382138No Hit
CAAACCGTACATACGACTTTCATCGAATACGGCTTTCCACAGAATTATAT20.14641288433382138No Hit
GTAACAACTAACATCTCATTTCAATAATATAATCATCAAATAGTCGTTAA20.14641288433382138No Hit
TCGTACATGTCGGATTAGTAGGCTTCTCGTATGAGATTGATACGGGGTCA20.14641288433382138No Hit
TACGACTAATCCACGACTAATGACAGTAGTCGTTATACCATAGTCGATAA20.14641288433382138No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGT20.14641288433382138No Hit
GGTTGCAATAATATTGGCCGTACTTGATGTCAACTGGTTGGTAGCAGATC20.14641288433382138No Hit
ATATACTCGACGTTAGATGGGTAACGATTTGTACTATTCAAATGTGGAGA20.14641288433382138No Hit
ATGTAGTAACACATGCGATCATTCCAGCACTAATGCACCGGATCCCATCA20.14641288433382138No Hit
GAAGTTGGAATCGTCGACCGGGTCCGAGAATTCGTCGACCAGGACGAGGA20.14641288433382138No Hit
GTAACAGTTAGTAACTAACCAAATTAGTGACAATCATGAGGGATTATTAA20.14641288433382138No Hit
TTTGGCTTTGTGTCTTCTAACAAGGATACAATTCTTACGCCTTTAAGATC20.14641288433382138No Hit
TGGTTGGGAATTTTCACTATATATTCGACTTCGTAGCAATGAGAGTTAGT20.14641288433382138No Hit
ACCCAACAGCTTCAAAACGTTCTTGTGACTGCTCATCATCGACAAAACCG20.14641288433382138No Hit
ATCTTGTAAAACAAACGAACAAAATGCTGAACTAATCTATCCAAACCGAG20.14641288433382138No Hit
AAAGCGCACTATGCTCCGTTTTAACAAATGAGAGTTTTCGGTGGAACCGG20.14641288433382138No Hit
CGAACTACACCTCACGTACATCGATATGAGATTGTTATTTGCGTACGGAT20.14641288433382138No Hit
ATTTGGAGGGATGATACATCGGTTTAAGGCAGGAGATACATCTCATATAG20.14641288433382138No Hit
ACGTCATTCTCACACCAGGGGAATTCAGTCGTCTGTTGGCAGAGTGTACG20.14641288433382138No Hit
ATTTTGGTTCTTCTTTCAGATGCAAAAGCGATGAATGAGTTTCAGCTATG20.14641288433382138No Hit
GAGTCCTGGGTCACGGTCACCACGCCGCCGTCCTCGAAGTTCATCACGCG20.14641288433382138No Hit
GCTTCATTCTCACTGCCAGCACTAAGAGCACCATTCGAAGCATCTTCCCA20.14641288433382138No Hit
ACGGAAGTGCATACCATGATTCTTCTGTCTATCAATAACAGCGTGCATTG20.14641288433382138No Hit
AAGTAAATTATAAACCAAAATGGTGTATTTACATAATGTGTAGTCTATGT20.14641288433382138No Hit
AATATCATGCTAAGAATAGCTAGGATTTCCAGAAGAAGATGCCATTCATT20.14641288433382138No Hit
AAGCAAAAAAGAACATAACCTCCGATACGATGAACAGAATAGAACCATAT20.14641288433382138No Hit
GATAGCGAACAAGTACCGCGAGGTAAAGATGAAAAGGACTTTGAAAAGAG20.14641288433382138No Hit

[WARN]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)