FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_AL_SPL9_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_AL_SPL9_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56
Sequences flagged as poor quality0
Sequence length76
%GC26

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATTGTATCTGAGCTGTACTGTAACCACAGGTACTCCCTCGAGTTCATA23.571428571428571No Hit
AAAAAAAATAGACAAAATATAACAAACAAATAAAAATTCAAAAATACAAA11.7857142857142856No Hit
TATAGTTTACAACTTTAGACAAACTTATAGATTGAAGATCTCAGTTTATA11.7857142857142856No Hit
AAACGGATTTATGATAATTTCGAAGTCAAATGATATATTAAATTTTCCAA11.7857142857142856No Hit
CAGTTAAAATCAGTCAAAAAACACATTTGATATAAATAGAGAACACAAGA11.7857142857142856No Hit
GTTCTGATTAAGAAATTCGTATGTTATGTTTTTTCTTCTGCCATAATGGT11.7857142857142856No Hit
ATCAACATACAAACATTTATCGAATTCATCATCGTCGTCGGCAATATCTT11.7857142857142856No Hit
AAAAGTTTGTTAAAGATATACGGTACTGTTTATACTGTAGATTGATCCTA11.7857142857142856No Hit
CCTCAAAAAGTTTTTACAGTTTTTTATTTTGGGATTGTAAAACAGTTTTT11.7857142857142856No Hit
AGGCAGTAATATCTGGCAAAGTAGCTGTTCGATTTCTCATTGGCCTTGTA11.7857142857142856No Hit
GTTACCAAACCTAACTACTCTTGTGTTGCTGCAGACGGTTGGGTGATTGA11.7857142857142856No Hit
TTTTTTTCTTTTAATATTGTAGGAAGATCTATGGCATATCATACCTATGC11.7857142857142856No Hit
AAGTAATATAATTAAATCAATCTTAATGTTAATTTATATATAATGATATA11.7857142857142856No Hit
CAAAGAAGAAGACAAATCTTCAGGCTTTTGTGAACGCACCTTATGGAGAT11.7857142857142856No Hit
AAAATTAATGATAATTTAATTAATAAACTAATTTGAAAAAAAAATGTTAA11.7857142857142856No Hit
TTTTTGAATATCAGTTAATTGTTTTAAATATTAAAGATATATGTTTTTGT11.7857142857142856No Hit
AAAAATTAACTAAAACAAAATTTAAGTTTAATATATTAAGTTAAGGTTTA11.7857142857142856No Hit
ATAACCTCTGATTAAGCTTCACTAAAATTAGATTAACAAACACATAAACA11.7857142857142856No Hit
AACTACAAACTTTAAATCATATATATAAAAATTAATATCTTACAATATAA11.7857142857142856No Hit
ATGGATTTCTCAAATTATAAAATAAATATAATAAAATGAAAAAATACAAC11.7857142857142856No Hit
TGGAAAGTGACTAGTCATCAATCATCATCATCAGTGCTAAGTACTTCAAT11.7857142857142856No Hit
TTTTATATTATTACTATAATTATAAAAAAGGAACTAAGAGATTCTTGGAA11.7857142857142856No Hit
CAAAACACCGAAAAATCCACTCAAAAGCAATGAAAATATATAAAAGACCA11.7857142857142856No Hit
TATGTAATAAATATTTTAAAGTCTAAAAAAAAAACAGATATCAAATATCA11.7857142857142856No Hit
ACCACAGAAAATGAATCTGCTAATGAAGGTTACAGATACGGGCAAGAATA11.7857142857142856No Hit
GTTATACTTAATATCGTTTTCAAAAAAAAAAAAAAAAACCATCAAAATTT11.7857142857142856No Hit
ATGGTGTTACAAAATAATAAAAAAAATCTAAATTTAAAAACTAATAAATA11.7857142857142856No Hit
AAGCATTCATTCGACTGAGGTAATAGATTAGTAATTGCCTTAAGACAAAC11.7857142857142856No Hit
AAAAATAAAAAAAAAAAAATACTAAAATATTCTAAAACAAAATAATAATA11.7857142857142856No Hit
AACAACTAATAATTATATATAAGATTGTCAAAATTCTTATCTGAATATAT11.7857142857142856No Hit
TTGAGTAAATACTGGCTTCCTCTGTTTCGACAATTAGAGGTTAATAATTA11.7857142857142856No Hit
CATGATTTTCCATCTCTCTCTCTGTGAATGTGATGGACCGATCAATGAGG11.7857142857142856No Hit
CTATATTAAAAAACAATAAAATGATTAGCCCAATTAATAATCAATATTTT11.7857142857142856No Hit
AAATATTTTAAAAATTGAATTTATATGAAACTTTTCTTTGGAAATCTGTA11.7857142857142856No Hit
GTATACATAATATTAAATAAAAACATAAAAAAATATTATAAAATTTTAGA11.7857142857142856No Hit
TTAAAACATTATTTTGATCATCACCAAACAATTATAATAGGAAAAGATCA11.7857142857142856No Hit
ATTATAACTAACTGTATTATCTCGTATTTGTTCAAAAAATACAGTAAAAA11.7857142857142856No Hit
CACTGACCAACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAA11.7857142857142856No Hit
AATCTTGACAAGTGAAATTTACTGGATTATTAGTTTTAGAGATAACATAT11.7857142857142856No Hit
ATATGTTCCATAAAAAAAAAAATAAATATAATTTTATACTTAATATAATT11.7857142857142856No Hit
AGATATAAAAACAAACGAATATTTAAATAAAATCACATCAGAAAAAAATT11.7857142857142856No Hit
TTTAGTAGTTTGTTTATGTTGGTTTTTGGTTATGGTTAACAAAATCAAAA11.7857142857142856No Hit
TTTATAGTCATATTTGACTATCATTTCTCTCATACATATAAAAAGATAGA11.7857142857142856No Hit
AACCTAATTAAAAAAAATATAAATACGCAATATCAAATATCAAATATAAA11.7857142857142856No Hit
AGTACAGTGCACAATTGCAACTACAAAACCAAATGTTGTCCCAGATGATC11.7857142857142856No Hit
AAAAAACAAAACAAAAATACACAAAAAACAATTGATGTTTTAATAAAAAT11.7857142857142856No Hit
CATCAAGCAGTTCGCAAGAGCCAACTAGATCCTCATCCCCAGGGATCAAT11.7857142857142856No Hit
ATTATTAAAAAGAAAGTTTTAAATTTTTAATAATATATTACGTTGAAAAA11.7857142857142856No Hit
AAAAACACCACCAACATAACCAACAACCCACAATATACGCCAAACAAAAT11.7857142857142856No Hit
AACGAGCTGAAGTAAGGATAAAAGGCCCTGGACTCGGAAGAGATGCGGCA11.7857142857142856No Hit
TAAGAGAAATTTTTTATTTGTAGATATATATTAATCTTTAAAAAAATTAA11.7857142857142856No Hit
AATCATTTTCTGTAACCACCCAATAGGTGTGGATGAACATAAAAGCCACA11.7857142857142856No Hit
TAATTTTTTCAGAAGAAAAAAAAAACATTAATATAATTATTATAAAAAAA11.7857142857142856No Hit
ATCAGCGGTTTGCAAGTAAAATTTCGTTGCATAATAAAACCGGAGTATAA11.7857142857142856No Hit
GCACTTGTACTTAGTCTAGAAGTATATCCGCAGTTCTTTGACTTCATTGC11.7857142857142856No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAATAC50.070.020
TAAATAA50.070.063
AAACAAA50.070.070
CCTAATT50.070.03
ACGCAAT50.070.025
TAAAAAC50.070.067
AAATATC50.070.035
TATAAAT50.070.018
TATAAAA50.070.045
AAATACG50.070.021
AAATAAT50.070.053
AAATAAA50.070.064
AATTAAT50.070.057
AATTAAA50.070.06
AACCTAA50.070.01
ATAATTA50.070.055
GCAATAT50.070.027
CAATATC50.070.028
TACGCAA50.070.024
ATTAATA50.070.058
Produced by FastQC (version 0.11.5)