FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_AGL104_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_AGL104_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3333789
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG45940.13780116258107517No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGCC2100.004008153511.66731556
TTACCAT23150.011.6526061
CGGACTG2600.001591924210.7732236
CGCCGTA2600.001597310610.76885959
TACCATA17300.09.9203952
GCAGCCG3955.535581E-48.86085254
CGTCCTA3200.0088831448.74956764
TTACTAT30900.08.6166491
AGCGACC3750.00339925828.4031146
TTACCAA33300.08.206041
CACGTCC3900.00486153648.076159562
AGCCGAC4600.002497137.609118556
TACCTTA14750.07.5986582
GTGTGCC12604.9112714E-117.22262370
CGCATCA5350.00136483797.19633754
TTACCTT25350.07.1863551
TACCAAA37350.07.12692072
TTGCCAT14351.4551915E-106.5916661
CAGGTCA5900.00382606826.525394448
ACGGCTC6000.00456904536.415482541
Produced by FastQC (version 0.11.5)