FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n02_AGL104_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n02_AGL104_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9973440
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG202810.20335009785991592No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCCGTA9900.015.91009259
GCCGTAT12400.014.39599560
GGTCGCC12200.011.7633956
TGGTCGC14000.010.75110755
CCGTATC16200.09.29042861
TCGGTGG19950.07.544107451
CGTATCA20150.07.46892862
AGGCTAT62350.07.01742270
TCGCCGT22000.07.000369558
GGGGGTA14603.274181E-116.712650366
GTCGCCG23200.06.638481657
TAGGCTA67600.06.420683469
CTCGGTG20900.06.36400550
AGTGTAG119400.06.302868470
TCGCGTG6250.00696870546.160634555
GTAGGCT71600.06.061986469
TGTAGGC78300.05.94560669
GTAGGCG8356.235109E-45.8687570
GCTATAG52650.05.78398469
GGCTATA62150.05.68835269
Produced by FastQC (version 0.11.5)