FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18038705
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372770.20665008934954032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG16750.018.5238841
TGGGGGG30600.018.114761
TAGGGGG6000.015.1017884
ATGCCGT16050.014.9823644
ATGGGGG10000.011.8490954
GAGACCG7250.011.08318231
CTCGTAT17400.010.47083444
ATCGGGG6402.0372681E-1010.40167342
CGCAGGC5107.508788E-810.35912770
TTAGGGG8700.09.6138713
TCTCGTA20650.09.16250443
CCAGTCA36850.08.99906626
CAGTCAC34250.08.76801427
CGAGACC8352.919478E-98.36683830
TATGGGG10557.2759576E-128.2583613
GATCTCG14100.08.19882941
TGCGCAT5552.7373422E-58.19704337
AATCGGG10202.237357E-107.899227641
GCGCATC4500.00200956227.77973638
AATGCGC6301.5909778E-57.770991335
Produced by FastQC (version 0.11.5)