FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG91_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG91_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8055103
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG15200.029.01185648
ATGCCGT15550.028.13378549
TGCCGTC15450.027.40977150
CGTATGC16300.027.05446
GCCGTCT15850.026.49723251
GTATGCC17500.025.19886847
CTCGTAT25400.017.22363744
TCGTATG26950.016.23303845
CGCTCAT27300.016.1531234
CCGCTCA27850.015.95978633
CCGTCTT28050.015.72121952
ACCGCTC29550.014.804749532
CTGCTTG29850.014.06972159
TCTCGTA33000.013.57514943
CACCGCT32500.013.56862231
TATCTCG34400.013.12441241
TCACCGC35500.012.3233930
GTCACCG36800.011.69784529
AGGGGGG12700.011.5829121
ATCTCGT39500.011.16405642
Produced by FastQC (version 0.11.5)