FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG91_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG91_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5885408
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG7400.017.02612542
CGGGGGT4350.016.09110344
TCGGGGG7100.011.83035943
AGGGGGG6950.011.5941381
AATCGGG12200.010.90106341
ACTCCGG14150.09.64613232
CACTCCG15100.09.03925631
GAATCGG15350.08.89203740
GCGGGGG3150.0078098148.88841952
CTCCGGA16450.08.72294233
TCCGGAG15650.08.72158234
TGGGGGG7553.3845936E-77.88432935
TCGGGGT5502.1918428E-47.635959643
GGGGGTG7301.4204976E-57.191445
CCGGAGA21150.07.115463335
CAGTCAC23900.06.736045427
GGGGGGT6853.6890263E-46.641984558
CCAGTCA23050.06.22526726
TCCAGTC24500.05.999682425
AGAATCG22200.05.99067439
Produced by FastQC (version 0.11.5)