FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG163_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG163_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5898523
Sequences flagged as poor quality0
Sequence length76
%GC33

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGT4050.019.8753844
ATCGGGG7550.019.0055142
TCGGGGG7250.015.447371543
ACTCCGG13650.012.05057732
CTCCGGA15150.011.5504833
AGGGGGG7000.011.5097681
CACTCCG14900.011.50939431
AATCGGG12700.011.29855241
TCCGGAG14300.011.25808234
CGCCTGT2500.001151569511.19953468
GAATCGG14100.010.42492240
CGGAGAA22500.08.86614836
CCGGAGA20100.08.53183935
GTCACTC21850.08.48920629
GGGGGTG6251.5673086E-68.39950845
GGGGTGT4250.00114641298.23502354
CAGTCAC23600.07.85964327
AGAATCG21900.07.83059239
CCAGTCA22050.07.77725726
TCACTCC23600.07.71141330
Produced by FastQC (version 0.11.5)