FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG163_col_B_l2-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG163_col_B_l2-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAAGAATGTTAATAGTGATAAGATTAATTAAATATATATACTTTTAAAA112.5No Hit
ATCATAAGGATACTAAATCCTATTTTCTGGTAAATTTTCATAATTTTTTG112.5No Hit
AGTGAGAGTCTCTCCGAAGATCGGAAGAGCACACGTCTGAACTCCAGTCA112.5Illumina Multiplexing PCR Primer 2.01 (100% over 33bp)
TTACGGAGGTCTCTCCTGAGATCGGAAGAGCACAGTCTGAACTCCAGTCA112.5No Hit
AAACAACTTTATATTTCCGGATCGAGACCGTGACTTTGGCCAAAGTAAAT112.5No Hit
ACTTGCATGTAACTGATAAAAATAATATTTGTTACTTTACGTATTTTGAA112.5No Hit
GAGGGGAGATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGATTCCAT112.5TruSeq Adapter, Index 7 (97% over 38bp)
CTTTCACTTTTAATTTTAAGTTCTTTATTTTGTTTTCGTAAGAAATTTTT112.5No Hit

[FAIL]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)