FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG163_col_B_l1-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG163_col_B_l1-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences921988
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC2207.2759576E-1222.27153638
TGCCGTC1301.0577818E-418.84514650
TGGGGGG1401.8582093E-417.508565
TGCGCAT2852.6739144E-1017.19206237
TATGCCG1503.159575E-416.3324648
ATGCGCA3005.5479177E-1016.3324636
ATGCCGT1554.0557093E-415.80560749
GCCGTCT1350.00294706315.55472451
AATGCGC3251.720764E-915.07611735
CGTATGC1450.004740641514.48198446
GTATGCC1450.004740641514.48198447
AGGGGGG1600.00904872913.1349841
GGCCACT1600.00909749213.12429823
TAATGCG3902.2302629E-812.56343134
CATCTCG2701.6721603E-411.66604241
CGCATCT3358.357703E-611.49192339
CTCGTAT2459.711948E-411.4279644
CAAGAGG2700.0021846110.36981645
CTAATGC6151.2708188E-79.10520433
GAGTGAT3100.0068400339.03177530
Produced by FastQC (version 0.11.5)