FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG162_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG162_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATTTAGATCGGAAGACACACGTCTGAACTCCAGTCACCGCTCATT125.0TruSeq Adapter, Index 2 (96% over 25bp)
TATGCTCTATTGGTGAATTCAGGAAATCAACTTGGTGTATGATAGGTGAT125.0No Hit
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTC125.0TruSeq Adapter, Index 2 (97% over 35bp)
TGAAAGACCCGGACAGAAGAGTCATACTACTACGGAAAGGGAAGTGAAAG125.0No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)