FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG162_r1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG162_r1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10117656
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCCGT11650.010.81493249
TATGCCG12300.09.95887148
GCCGTCT12350.09.63530851
TGCCGTC12450.09.55777450
GTATGCC14300.08.81076647
CGTATGC14100.08.43931146
ACGGGGG4350.00144289398.04571151
ACCCCCG4854.796016E-47.93795965
CTCGTAT22150.07.426279544
CGCTCAT17200.07.325193434
CCGCTCA20350.06.5352833
AGGGGGG15351.10958354E-106.38917351
GCGTCGG7650.00140225215.947427346
CGGACTG12152.6249945E-65.76403146
ACCGCTC22001.8189894E-125.726969732
TCGTATG24300.05.617014445
CACCGCT23700.05.611517431
CCGTCTT24850.05.492775452
CGCGTAA7700.0097636335.45425730