FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG162_col_B_l2-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG162_col_B_l2-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5677383
Sequences flagged as poor quality0
Sequence length76
%GC32

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5601.8189894E-1212.49943150
TATGCCG6551.8189894E-1211.22086248
CGCCTGT2250.00670837510.88839368
GCCGTCT6251.5079422E-910.0795451
CGTATGC7302.2191671E-109.58860446
ATGCCGT9051.8189894E-129.28134549
TGGGGGG6452.6292764E-78.6857535
GTATGCC7352.438901E-88.57103947
ACAGGAC5050.0062110466.930377537
CTCGTAT11501.4748002E-76.39101344
TATCTCG15001.7957063E-85.83306841
CCGTCTT10951.7998349E-55.75316352
GTCACCT14051.7378807E-75.72927629
CCAGTCA15853.4524783E-75.299443226
CTGAAGC15309.563555E-75.261198534
CCTGAAG14005.672364E-65.24976133
CTTCTGC14802.8253453E-65.202465556
TCGTATG14156.885566E-65.1941145
AGCTATC13702.1670685E-55.109256338
GCTATCT13752.3079674E-55.090677339
Produced by FastQC (version 0.11.5)