FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG162_col_B_l1-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG162_col_B_l1-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1808500
Sequences flagged as poor quality0
Sequence length76
%GC31

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG1207.3536285E-826.2724551
CGGACTG901.9226407E-423.3455436
TGCCGTC1951.8291757E-819.74244950
GCCGTCT2207.283961E-817.49899151
TATGCCG2251.7652928E-615.55465948
CGTATGC2352.7748138E-614.89275846
GCCGTGA1500.005938154613.99957970
GTATGCC2505.270458E-613.99919347
AGACCGG1550.00737938213.547605533
CTGCTTG2952.0213647E-613.05009559
CTCGTAT3651.7262355E-611.506186544
ATGCCGT3054.070007E-511.47474949
CGCAATC2150.004780336311.39469139
CCGTCTT3702.5164678E-510.40480652
TTCCAGG3105.9601106E-410.1699841
CGTCTTC5151.037366E-69.51401453
CCTGGGA3100.0068455839.03173755
CTTCTGC4552.4090594E-48.46105156
TCGTATC5653.4133955E-58.05263338
GTCACGA6805.207645E-67.72014329
Produced by FastQC (version 0.11.5)