FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG141_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG141_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4514659
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6350.019.84158950
TATGCCG6300.019.44353348
GCCGTCT6850.018.39411451
ATGCCGT7450.016.91195949
GTATGCC7950.015.84831447
CGTATGC10100.013.86073846
CGCTCAT9850.012.79142234
CCGCTCA10300.011.55298433
ACCGCTC11150.010.98615532
CCGTCTT13600.09.77938752
CTCGTAT14700.08.80911244
TCGTATG16550.08.67028845
CACCGCT14900.08.45607431
TCACCGC15750.08.44414330
GTCACCG15800.08.4174229
AGGGGGG7503.3953256E-88.4056591
CTGCTTG16300.08.15939859
TACGCAG4804.292727E-48.020102515
TCTCGTA20950.07.18343443
TATCTCG18850.06.86970541
Produced by FastQC (version 0.11.5)