FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG141_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG141_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2587161
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4750.030.20866848
ATGCCGT4900.029.28391349
GTATGCC5000.028.69823347
GCCGTCT4850.028.14259751
TGCCGTC5050.027.72106650
CGTATGC5350.026.82077846
AGGGGGG4450.019.6807291
CTCGTAT7750.018.5149944
CGGACTG2852.6557245E-1017.2028966
CCGTCTT9250.016.26926852
TCGTATG9550.015.39172245
TCTCGTA9800.014.64195643
CCTGACG1600.00910379413.12419226
CTGCTTG10550.012.937592559
GGACTGT4153.919922E-912.6498237
CTTCTGC12500.012.03925956
CATTCAG15600.011.66594833
CAGTCCG2751.9859846E-411.4538427
GACTGTA5259.1495167E-1011.3326358
CGTAGTG2250.00670750710.88821930
Produced by FastQC (version 0.11.5)