FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG138_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG138_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1608396
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC2650.025.09323750
GCCGTCT2850.022.10429251
ATGCCGT3350.021.93933549
TATGCCG3250.021.53751448
GTATGCC3150.021.11018447
CGTATGC3600.018.4714146
CGCTCAT4100.017.9260434
CTCGTAT4400.015.9083944
CCGCTCA4850.015.15397333
ACCGCTC5500.013.99938432
CCGTCTT5350.013.08353752
TCTCGTA5650.013.00827843
CACCGCT5650.013.00827831
CTGCTTG6000.012.83276959
TCACCGC6500.012.3840730
TCGTATG6159.094947E-1211.38161345
CCCAGCA2250.006704769610.8884136
CGGACTG2600.001590462510.7737816
TATCTCG7401.8189894E-1210.40494841
CGTCTTC7453.6379788E-1210.33511653
Produced by FastQC (version 0.11.5)