FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG138_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG138_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2122198
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT3900.027.8190951
TATGCCG4050.027.65290848
GTATGCC4200.027.49859247
TGCCGTC4250.027.1750850
CGTATGC4400.026.24865746
ATGCCGT4500.024.88761549
CTCGTAT6150.018.2104544
TCGTATG6900.016.73827645
CCGTCTT7450.015.97233852
CATCTCG8050.013.91233341
CTGCTTG7850.013.82094959
AGGGGGG4455.638867E-1113.38145351
CTTCTGC9750.012.92241556
TCTCGTA9500.011.42046843
ATCTCGT10600.010.56549742
CGTCTTC11350.010.17569153
CACGAGA12450.09.27663331
TGCTTGA13650.09.23029760
AGTCACG11850.09.1556528
TCTGCTT14700.09.04715658
Produced by FastQC (version 0.11.5)