FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG137_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG137_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3770544
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC8050.036.08531650
GCCGTCT8400.034.5822251
TATGCCG8700.033.7915748
ATGCCGT9150.032.1296949
GTATGCC9500.030.94596147
CGTATGC10350.028.40450746
CCGTCTT11850.024.51397952
CTCGTAT13000.023.15279444
CTGCTTG13150.022.09025259
TCGTATG14600.020.37578445
CGTCTTC15850.019.65236553
TCTCGTA15300.019.21481343
CGCTCAT15200.019.11097334
CCGCTCA15450.018.80173533
AGGGGGG5250.018.6811751
ACCGCTC16000.018.37416532
TATCTCG16300.018.25070641
CACCGCT17800.016.51610231
CTTCTGC18200.016.15332656
TCACCGC20300.014.482130
Produced by FastQC (version 0.11.5)