FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_SG137_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_SG137_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3763534
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC5800.030.7745550
TATGCCG6200.028.78909548
GCCGTCT6100.028.687351
ATGCCGT6550.026.71641549
CGTATGC7350.025.23701946
GTATGCC7850.023.18372347
AGGGGGG4750.017.6970941
CCGTCTT9900.017.32249352
CTCGTAT11200.016.56179244
CTGCTTG10900.015.09109959
TCGTATG13050.014.75032945
TCTCGTA13350.013.89453843
CCGCTCA12900.013.83661833
CGCTCAT12950.013.783195534
CTTCTGC13600.013.63912356
TATCTCG13750.012.98126441
CGTCTTC14400.012.39530453
ACCGCTC14900.011.97935432
CACCGCT16450.010.63784331
ATCTCGT19950.09.29784942
Produced by FastQC (version 0.11.5)