FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_NAC_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_NAC_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3761744
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT41550.11045408725314641TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGTCT8550.032.74691851
TGCCGTC9250.031.78220750
TATGCCG9500.031.6826448
GTATGCC9900.030.40253447
CGTATGC10000.029.39854246
ATGCCGT10000.029.39854249
CCGTCTT12950.022.4313252
CTCGTAT13800.021.81051344
TCGTATG13750.021.6352945
TCGTATC18400.018.8305938
TATCTCG16750.018.8050441
TCTCGTA16750.018.3871543
AGGGGGG7200.017.5128671
CTGCTTG14900.016.67702759
CGTATCT18900.016.6658439
ATCTCGT21800.015.09099642
CGTCTTC19450.014.93499253
GTATCTC22450.014.49816940
TTCGTAT26650.013.26388337
CTTCTGC20350.013.24258756
Produced by FastQC (version 0.11.5)