FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_MYC2_r2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_MYC2_r2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17997287
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25950.021.98359148
TGCCGTC28200.020.60190450
ATGCCGT28600.020.43613849
GCCGTCT29700.018.73663151
CGTATGC31650.018.1350446
GTATGCC36750.015.99927647
TCGTATG44950.013.002748545
CGCTCAT46450.012.58285434
CTCGTAT52350.011.09787444
CCGTCTT56300.010.25714352
TATCTCG58550.010.10204741
CCGCTCA59850.09.82411833
CACCGCT60750.09.50574231
ACCGCTC61700.09.41610632
TCTCGTA66400.08.9077343
GTCACCG64200.08.77686129
TCACCGC65000.08.77652630
ATCTCGT81250.07.71044242
AGTCACC80950.07.52280228
CAGTCAC91200.06.638843527