FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_MYC2_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_MYC2_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7575866
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT89480.11811190958235006TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19350.037.62092248
TGCCGTC20250.035.94888350
GCCGTCT21300.034.34106451
ATGCCGT21900.033.24040649
GTATGCC22200.032.6335647
CGTATGC22150.032.54921746
AGGGGGG11100.027.4533941
CCGTCTT29850.024.85642252
CTCGTAT30300.023.67872444
CTGCTTG30100.023.48739259
GATCTCG30700.023.02820441
TCGTATG31800.022.89197545
TCTCGTA34200.020.67151643
CGATCTC34550.020.25951440
CGTCTTC37100.019.7160353
CTTCTGC39150.018.6837756
CTCGATC45150.015.96822138
ATCTCGT48350.015.27330742
TTACTCG48850.014.68711135
TTCTGCT51150.014.5057557
Produced by FastQC (version 0.11.5)