FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_LBD10_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_LBD10_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147257
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC4300.031.74239250
ATGCCGT4100.031.5835849
GCCGTCT4000.031.49821951
TATGCCG4400.030.22556348
CGTATGC4650.027.84788746
GTATGCC4950.026.86716847
CTCGTAT6400.021.3269244
AGGGGGG3200.019.7065771
CCGTCTT6600.019.0898352
TCGTATG6850.018.9040445
CTGCTTG8550.015.55467659
GCTATCT9150.014.53469839
TATCTCG9400.014.52045541
TCTCGTA8950.014.46845543
CGTCTTC10000.013.29924953
CTTCTGC11100.012.927196556
GTACACG2203.9334796E-412.72655362
ATCTCGT10850.012.57993442
AGCTATC10300.012.57210538
TGGCGAC2150.004780791711.39470536
Produced by FastQC (version 0.11.5)