FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_HDL_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_HDL_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11736446
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG17550.020.34089742
CGGGGGT8950.020.33421344
TCGGGGG14650.018.87280843
CACTCCG35100.010.8684231
AATCGGG35750.010.67081341
CGGGGTG6704.3655746E-1110.44725553
GCGGGGT7051.0913936E-1110.42502752
GGCGGGG8450.010.35452851
GAATCGG37450.010.18642340
GTCGCGG4501.916229E-610.11066562
ACTCCGG38600.09.97361132
GGGTGGC10300.09.17431447
GGGGGTG17050.09.0318245
TCCGGAG44300.09.00633634
GCGGGCG3904.8798957E-48.97392565
CTCCGGA44300.08.69032533
CCGGAGA45750.08.56789235
CGACCCC16000.08.53083768
CGGGGGG12150.08.06545444
GTCACTC49050.08.06281129
Produced by FastQC (version 0.11.5)