FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_HDL_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_HDL_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11761639
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG17050.019.29522742
CGGGGGT9400.018.98849744
TCGGGGG15200.017.03862643
AATCGGG34650.010.70653741
CACTCCG37200.010.53715431
GAATCGG36150.010.26228240
ACTCCGG37950.010.23668832
TCCGGAG46550.08.9469634
CTCCGGA44500.08.88723633
GGGGGTG14350.08.78008745
CCGGAGA48050.08.6676635
GCGGGGT6603.7606333E-78.484570552
AGGGGGG19200.08.3913841
TGCGGGG5552.7333515E-58.19792951
GTGGGGG12200.08.03252357
GTCACTC50800.07.991762629
CGGGGTT10605.14774E-107.593993744
CGGGGGG11901.0186341E-107.352606344
TCACTCC54500.07.2565530
CAGTCAC53850.07.149041727
Produced by FastQC (version 0.11.5)