FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_HB26_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_HB26_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2361103
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCATC5900.023.72772838
TGCCGTC3150.023.33226850
TGCGCAT7050.021.34654237
GCCGTCT3450.020.28892951
ATGCGCA7400.019.86395836
TATGCCG3650.019.17720448
AATGCGC7950.018.48972135
ATGCCGT3900.017.94789949
AGGGGGG4100.017.0839671
GTATGCC4400.015.90836347
CGTATGC4201.8189894E-1214.99931546
CGCATCT8300.014.75836239
CCGTCTT5600.014.37434252
TAATGCG10300.014.27119234
CATCTCG7650.013.26736741
CTCGTAT5652.0008883E-1111.76937444
TCGTATG5905.529728E-1010.67747845
CTAATGC15200.010.36136833
TCTCGTA7757.2759576E-129.93503143
GCATCTC10150.09.65473240
Produced by FastQC (version 0.11.5)