FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_HALO-bZIP53_eGST-bZIP63_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_HALO-bZIP53_eGST-bZIP63_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences190339
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTCCGGAGAATCGGGGGT2700.14185216902474007TruSeq Adapter, Index 6 (97% over 36bp)
AGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1930.10139803193249938No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCCTT200.006943273452.49980545
TCTACGG200.006943273452.49980551
GGACCCT200.006943273452.49980539
CGGGGTT350.001253153439.9998544
TCGGGGG801.4115358E-939.37485543
CGGGGGT556.669974E-638.18167544
TCGGGGT400.002414425434.9998743
TGGGGGG501.7051733E-434.99986650
ATACGCT450.004299631.11099466
TTCTGGC450.004299631.11099455
AGGGGGG802.4544188E-630.6248861
ATCGGGG1500.030.3332242
ATAGCAG500.007195628227.99989360
AAAGCGT500.007195628227.9998937
AATCGGG1750.027.99989341
CGAAGCC500.007195628227.99989332
AGAATCG2000.026.249939
GAATCGG2051.8189894E-1223.9023540
CGGAGAA2750.021.63628236
GGGGGTG1003.9171183E-420.9999245
Produced by FastQC (version 0.11.5)