FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_HALO-CCA1_eGST-LHY1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_HALO-CCA1_eGST-LHY1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3779396
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAATTCGTATCTCGTAT41620.11012341654592427TruSeq Adapter, Index 7 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG9150.032.51158548
ATGCCGT9450.031.4794749
GCCGTCT8800.031.4192851
GTATGCC9850.030.91173447
TGCCGTC9600.030.62304350
CGTATGC10400.028.94045846
CTCGTAT14500.021.24002344
TCGTATG14000.020.9986645
CCGTCTT14600.020.13623252
TATCTCG17300.018.61152841
CTGCTTG15050.018.13861559
TCTCGTA18750.016.61227243
CGTATCT19800.016.43834339
TCGTATC21850.016.3376338
ATCTCGT21550.015.59065142
AGGGGGG7250.014.9792261
CGTCTTC19100.014.84237753
GTATCTC22750.014.46061440
CTTCTGC19850.013.75263456
ATTCGTA27050.013.71446636
Produced by FastQC (version 0.11.5)