FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DUO3_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DUO3_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2019586
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC3200.033.90445350
ATGCCGT3500.030.99835649
GCCGTCT3250.029.0753851
TATGCCG3700.028.37687148
GTATGCC3850.027.27127847
CGTATGC4200.024.99867246
TCGTATG6400.016.4053845
CCGTCTT6300.016.11025652
CTCGTAT6550.016.02968444
CTGCTTG6350.015.43225259
TCTCGTA8300.013.4932643
CCGACCT1900.001894001812.894052521
ATCTCGT9700.012.26739142
CGTCTTC9650.011.96827753
AATCTCG9700.011.90658641
CATTCAG12400.010.44299433
CTTCTGC9650.010.154901556
AGTGGCC3208.010187E-49.84810453
CAGTCAC14000.09.24950927
CCAGTCA15900.08.80456426
Produced by FastQC (version 0.11.5)