FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DUO3_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DUO3_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1220349
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT12760.10456025284570235TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2700.034.9991247
TATGCCG2850.033.1570648
TGCCGTC2800.032.4991850
GCCGTCT2900.031.3785251
CGTATGC3450.026.37614846
ATGCCGT3600.026.24933849
CTCGTAT3850.022.72670244
CCGTCTT4350.021.72359352
AGGGGGG2201.5643309E-1020.6906221
TCGTATG4750.019.15741245
TCTCGTA4950.017.67632343
CTGCTTG5200.017.4995659
GCGAGTG1000.00969210717.4995635
GATCTCG5650.016.10578741
ATCTCGT5650.015.48633542
CGGTCGG1400.00375472814.99962266
CTTCTGC6000.014.58296656
CGTCTTC6700.014.10412253
CGATCTC6850.013.79527340
TTACTCG7050.012.90747735
Produced by FastQC (version 0.11.5)