FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DUO1_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DUO1_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2690583
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCA1450.00474376714.4819956
AGGGGGG3655.675247E-1014.3948361
TATGCCG4001.5097612E-1013.99925848
CTGCGTA1800.001255250913.6179813
GCCGTCT3951.877197E-913.29043451
ATGCCGT4103.272362E-912.80419849
TGCCGTC4903.748937E-911.42796550
CGTATGC4652.116758E-811.28972346
AACCCCC5106.9230737E-910.9889981
GTATGCC4803.3795004E-810.9369247
AACCGCG3500.00181139989.0070541
ACTTGCG3600.00234416228.7550682
AACTATA34850.08.5432761
AACGACC6957.015542E-67.55987741
CTCGTAT7058.687164E-67.446413544
CATCTCG7058.687164E-67.446413541
CCGTCTT8251.3927238E-67.21173852
AACGGGC4950.0050722537.0762491
AACTGTG10058.54925E-86.97063351
AACTGCA24800.06.9207431