FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DUO1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DUO1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences997689
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCCCC853.959609E-628.82231369
CCCTGCG1107.4757764E-419.0910653
ATGCCGT1651.5941314E-619.08914649
CGTCCCC1250.001762871216.79929268
CCCCTCC1300.00229039316.15397355
AGGGGGG1807.084838E-515.5720681
CCGTCCC1656.518672E-414.84860267
GTTACGG1550.00737802913.54713527
GTCCCTG2102.6512178E-413.33343951
CCCAGTC1850.001547880413.24334861
TCCCCCG1600.00909449413.12510370
CGTTCCC2203.9280593E-412.72737457
CTCGTAT2952.892958E-511.86331144
TCCGTCC2150.004775440311.39543866
GGACTGT2150.004779012411.3942977
GCCGTTC2300.007887009510.65225955
GCTCCCG2850.0034219959.82365343
GTGGATA3150.00780288278.88806810
TCGTATG3650.00266962688.6293445
GAGCCGT3850.0043189718.18188353