FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ3_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ3_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17961000
Sequences flagged as poor quality0
Sequence length76
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG25400.020.53040948
ATGCCGT28700.018.657649
TGCCGTC28600.018.35572250
CGTATGC29300.017.55879846
GCCGTCT31050.016.56921651
TCGTATG37600.014.14818745
GTATGCC38800.013.71061547
CGCTCAT46300.011.56529434
CTCGTAT47200.011.122292544
CCGTCTT53050.010.09374452
CACCGCT57200.09.48376531
TATCTCG58000.09.29263741
TCTCGTA62250.09.0517343
CCGCTCA61100.08.99300233
ACCGCTC63600.08.80458932
GTCACCG62150.08.44685729
TCACCGC63150.08.3685230
ATCTCGT82700.06.98272142
AGTCACC80500.06.86920128
CAGTCAC87750.06.182008727
Produced by FastQC (version 0.11.5)