FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ3_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ3_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7593846
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT89600.117990277917145TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG19550.036.340948
GCCGTCT21000.033.3321151
TGCCGTC21600.033.21593550
CGTATGC21700.032.90158546
GTATGCC21600.032.8918847
ATGCCGT22000.032.29384649
CCGTCTT27950.025.66989952
CTCGTAT27800.024.67502844
TCGTATG31000.023.25690545
CTGCTTG29150.023.17208559
GATCTCG31350.021.6574741
CGATCTC34000.020.17533140
TCTCGTA34350.019.56221643
AGGGGGG11700.018.860861
CGTCTTC38950.018.3305253
CTTCTGC38050.018.2122156
CTCGATC46000.014.91220238
TTACTCG48950.014.79998235
TCGATCT47000.014.52045639
TACTCGA51550.014.18930636
Produced by FastQC (version 0.11.5)