FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ2_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ2_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9950432
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG40400.035.60456542
AATCGGG52750.028.13122241
CGGGGGT24000.027.56091344
TCGGGGG37700.027.29285843
GAATCGG63250.023.90388340
CGGGGTG10000.021.34887953
AGGGGGG22000.020.6985171
AGAATCG78400.020.22215339
TCGGGGT19350.019.89549643
TCCGGAG88450.018.55753134
TGCGGGG10000.017.84906251
ACTCCGG91700.017.7471632
CCGGAGA94300.017.5547535
CGGGGTT20400.017.49899344
CGGAGAA95200.017.27850336
CTCCGGA94850.017.1577733
CACTCCG97250.016.73425531
GCGGGGT13450.016.653452
GGAGAAT105650.015.40382837
CGGGGGG24250.015.15376844
Produced by FastQC (version 0.11.5)