FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ2_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ2_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3341502
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC6200.038.9495850
GCCGTCT6550.037.4031951
TATGCCG7450.033.35396248
ATGCCGT7750.031.61125449
CGTATGC7750.031.15966446
GTATGCC8150.029.63035647
CTCGTAT10300.023.4453844
TCGTATG10900.023.11806145
CCGTCTT11050.021.85439152
CTGCTTG11150.021.65871259
CGATCTC12700.018.73918240
TCTCGTA13050.017.9684143
GATCTCG13300.017.89380341
ATCTCGT15450.015.17720242
TTACTCG16650.014.71394735
CGTCTTC17700.013.6435653
CTTCTGC18300.013.3874856
CTCGATC19450.012.95562338
AGGGGGG7050.012.91795351
TCGATCT21500.012.5342339
Produced by FastQC (version 0.11.5)