FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ1_col_B_l-c.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ1_col_B_l-c.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13667858
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT272320.19924116858691393TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50200.042.17909648
TGCCGTC52100.040.57386850
GCCGTCT52350.039.91212551
ATGCCGT53350.039.75441749
CGTATGC54650.038.48840746
GTATGCC57150.037.04970647
TCGTATG65400.032.3224945
CTCGTAT68650.030.02758444
CCGTCTT72800.029.27744152
TCTCGTA86050.023.83374843
GATCTCG84550.023.8014341
AGGGGGG25850.022.3571011
CGATCTC91200.022.21941240
CGTCTTC102000.021.03330453
CTGCTTG99850.020.92546359
ATCTCGT98100.020.51387242
TCGATCT121450.017.05976339
TGCTTGA128050.016.56310860
TTACTCG131700.016.39632835
CTTCTGC129400.016.30916856
Produced by FastQC (version 0.11.5)