FastQCFastQC Report
Fri 8 Nov 2019
HW7CJAFXY_n01_DAZ1_col_B_L.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHW7CJAFXY_n01_DAZ1_col_B_L.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13702189
Sequences flagged as poor quality0
Sequence length76
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTACTCGATCTCGTAT272600.1989463143443723TruSeq Adapter, Index 27 (97% over 39bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG50100.043.10159348
TGCCGTC49800.042.86929750
GCCGTCT50900.041.94331451
ATGCCGT52400.041.0761549
CGTATGC52750.040.7372646
GTATGCC57550.037.6436147
TCGTATG63250.034.2512245
CTCGTAT66750.030.93475244
CCGTCTT73850.029.43010152
GATCTCG83800.024.55739841
TCTCGTA86600.023.8440543
CGATCTC90650.022.58588440
CGTCTTC98400.022.05196453
ATCTCGT97500.021.2862542
CTGCTTG100950.021.252459
AGGGGGG25350.017.6864621
TCGATCT118600.017.55825439
TTACTCG129500.016.8910335
CTTCTGC130750.016.86369356
TGCTTGA129850.016.73799760
Produced by FastQC (version 0.11.5)